q-PCR Methodology for Monitoring the Thermophilic Hydrogen Producers Enriched from Elephant Dung

This study aims to create a quantitative polymerase chain reaction (q-PCR) methodology for monitoring the hydrogen-producing mixed cultures enriched from elephant dung using alpha-cellulose as carbon source through five generations of repetitive sub-culture. The thermophilic fifth cultivation cycle gave highest hydrogen yield 170.3 mL H2/g cellulose and were used generate sawdust. Clostridium sp. Thermoanaerobacterium dominant bacteria in with high yield, according reaction-denatured gradient gel electrophoresis (PCR-DGGE). q-PCR primers Chis150F ClostIR, TherF TherR, BacdF BacdR developed amplify 16S rRNA genes sp., Bacillus respectively, quantification biohydrogen fermentation. Similar analysis gene amplification during production sawdust revealed increasing copy number time. molecular approaches this can be monitor microbial communities fermentation processes efficiently.